(LB Agar)Luria Bertani Agar 500gm

Luria Bertani Agar, Miller ( Miller Luria Bertani Agar)

Description:

LB Agar recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms.

Composition:

Ingredients Grams/Litre
Tryptone 10.000
Yeast extract 5.000
Sodium chloride 10.000
Agar 15.000
Final pH (at 25°C) 7.5±0.2
** Formula adjusted, standardized to suit performance parameters
Directions:

Suspend 40 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired. Mix well and and
pour into sterile Petri plates.
Principle and Interpretation:
LB Agar is prepared as described by Lennox (4) for cultivation and maintenance
of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (1) is slightly
different with double amount of sodium chloride. The media is nutritionally rich for the
growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12
are deficient in Vitamin B synthesis are further modified by specific mutation to create
auxotrophic strains and are therefore unable to grow on nutritionally deficient media.
Tryptone provides peptides and peptones while Vitamin B complex is provided by yeast
extract. Sodium chloride provides sodium ions for membrane transport and also maintains
the osmotic equilibrium of the medium.