cDNA Synthesis Kit 100rxn

ABscript II cDNA First Strand Synthesis Kit

Description of cDNA Synthesis Kit: 

cDNA Synthesis Kit features two optimized mixes, ABScript II Enzyme  Mix and ABScript II Reaction Mix. The enzyme mix combines ABScript II Reverse Transcriptase and RNase Inhibitor, and the reaction mix contains an optimized buffer.
ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced  RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, which provides higher specificity and higher yield of cDNA.

Specification: 

ABScript II Enzyme Mix        200 µl
ABScript II Reaction Mix       1.25 ml
Oligo d(T)23VN * (50 μM)     200 µl
Random Primer Mix (60 μM  200 µl
dNTPs (10 mM each)                100 µl
Nuclease-free H2O                    1.25 ml